The streak plate technique is the most popular method for isolating specific bacteria from a sample containing a mixture of microorganisms. Refer to Aseptic techniques before starting this or any other microbiology practical work. You can make streak plates of bacteria or yeasts. Jump to Technique - In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.‎Growth medium · ‎Incubation · ‎Importance.


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The experiments streak plate technique in Biochemistry Virtual Lab I are fundamental in nature, dealing with the identification and classification of various carbohydrates, acid-base titrations of amino acids, isolation of proteins from their natural sources, etc.

Streaking (microbiology)

Biochemistry Virtual Lab II Biochemistry Virtual Lab II deals with topics like streak plate technique, purification of plant pigments and natural products as well as estimation of iodine value and saponification value of fats and oils.

Population ecology Virtual Lab I A population is a collection of individuals of the same species that live together in a region.


Population ecology is the study of populations especially population abundance and streak plate technique they change over time. Crucial to this study are the various interactions between a population and its resources. Studies on simple models of interacting species is the main focus this simulation oriented lab.

Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab

Population ecology Virtual Lab II Population ecology is the study of populations especially population abundance and how they change over time. Studies based on models of predation, competition as seen in interacting species is the main focus this simulation oriented lab.

The bacteria growth can be supported by this streak plate technique greatly due in part to the high amounts of nutrients.


Choice of which growth medium is used depends on which microorganism is being cultured, or selected for. Different labs have different standards as to the direction and style of the streaking. Incubation[ edit ] Dependent on the strain, the plate may then be incubatedusually for 24 to 36 hours, to allow the bacteria to reproduce.

At the end of incubation there should be enough bacteria to form visible colonies streak plate technique the areas touched by the inoculation loop.

Isolation Techniques for a Streak Plate | Sciencing

Automated equipment is used at industrial level for streak plating the solid media in order to achieve better sterilization and consistency of streaking and for reliably streak plate technique work.

While streaking manually it is important to avoid scratching the solid medium as subsequent streak lines will be damaged and non-uniform deposition of inoculum at damaged sites on the medium yield clustered growth of microbes which may extend into nearby streak lines.

Importance[ edit ] Bacteria exist in water, soil and food, on skin, and intestinal tract normal flora. At all times hold the loop as still as possible. Make sure that a small amount of the culture is carried over.

Flame the loop again. Streak swab of clinical sample over one quarter of the sterile Petri dish in a back-and-forth "tornado" pattern.

This is quadrant 1. It allows microbiologists to distinguish and isolate individual bacterial colonies.

A colony is a visible cluster of bacteria. All of the bacteria in a single colony originate from the same bacterial cell.

Making a streak plate

The pure colony is transferred to another plate to produce a pure streak plate technique consisting of one type of bacteria. Sciencing Video Vault Procedure When streak plate technique properly, streak plate isolation thins out a specimen and enables individual bacterial cells to develop into isolated colonies.

A microbiologist begins by sterilizing the inoculating loop in a flame.