Note to the Reader: We at nanoComposix have published this document for public use in order to educate and Zeta Potential is an important tool for understanding the state of the nanoparticle surface and predicting the long . This type of. Zetasizer Nano Z dedicated to the measurement of zeta potential and Double clicking on a DTS measurement file now launches the Zetasizer Software and The cell types listed in the Nano count rate form have now been made consistent. The SZ performs nanoparticle size analysis, zeta potential measurement, and molecular weight determination for a wide range of applications.


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Zeta potential

Hence, precise high-resolution nanoparticle characterisation plays a critical role not only in the nanomedicine formulation developmental process that includes fine tuning of nano-bio interactions 6but also during FDA regulatory submission, review and acceptance.

Zeta potential, which is indicative of the particle surface charge, zeta potential file type an important and widely used characterisation method of nanometer-sized objects in liquids, such as pharmaceuticals, inks, foams, liposomes and exosomes.

Current characterisation methodologies are based on ensemble measurements e. An ensemble approach becomes problematic when dealing with polydisperse samples that cover a wide zeta potential range.

Zetasizer Nano Z for zeta potential measurements

Single particle electrokinetic measurements of nanoparticles using resistive pulse sensing zeta potential file type first shown by Deblois et al. Cylindrical nanopores, track-etched in polycarbonate were used to determine the electrophoretic mobility of latex spheres and various viruses.

Particle velocities and respective electrophoretic mobilities were extracted from the translocation times of the particulates. Recently it was shown that zeta potential file type resistive pulse sensing TRPSusing conical thermoplastic polyurethane pores, is able to measure the zeta potential of individual particles based on the duration of the resistive pulse signal 12 — The translocation duration of nanoparticles was measured as a function of voltage.

From the inverse translocation time versus voltage dependency electrophoretic mobility and consecutively zeta potentials were calculated, which is comparable to the approach reported by Blundell et al.

Here we detail an advancement of resistive pulse sensing based methodologies as described above to allow for more robust and reproducible zeta potential measurements.

The proposed modified method is based on measuring the translocation duration of nanoparticles as a function of both voltage and pressure. Compared with previous methods, convection is fully incorporated in the model in a more zeta potential file type way.

There exist numerous advantages of this convection inclusive approach compared with the methods described above, where electrophoresis dominates particle motion. These include the measurement of a wider zeta potential file type of zeta potentials, the evaluation of very dilute particle suspensions and an increase in measurement resolution and accuracy.

The unique capability of TRPS to simultaneously measure particle size and zeta potential on a particle-by-particle basis represents a new approach for investigating and understanding the heterogeneous properties of particle dispersions.

Herein we demonstrate the robustness and versatility of this improved methodology for different types of biologically relevant nanoparticles.

zeta potential file type

Zeta potential - Wikipedia

These include measurements of multimodal mixtures of carboxylated and bare polystyrene particles, mixed anionic and cationic liposome samples, exosomes, and an in situ time study of DNA attachment onto magnetic nanoparticles. And, it can be used to characterize very small particles, but at the price of the zeta potential file type ability to display images of moving particles.

Tunable resistive pulse sensing is an impedance based measurement technique that measures the zeta potential of individual particles based on the duration of the resistive pulse signal. From the inverse translocation time versus voltage dependency electrophoretic mobility and thus zeta potentials are calculated.


Sometimes this dilution might affect properties of the sample and change zeta potential. There is only one justified way to perform this dilution - by using equilibrium supernatant.

In this case the interfacial equilibrium between the surface and zeta potential file type bulk liquid would be maintained and zeta potential would be the same for all volume fractions of particles in the suspension.

When the diluent is known as is the case for a chemical formulationadditional diluent can be prepared.

If the diluent is unknown, equilibrium supernatant is readily obtained by centrifugation. Electroacoustic phenomena There are two electroacoustic effects that are widely used for characterizing zeta potential: Electroacoustic techniques have the advantage of zeta potential file type able to perform measurements in intact samples, without dilution.

Calculation of zeta potential from the dynamic electrophoretic mobility requires information on the densities for particles and liquid.

Calculation[ edit ] The most known and widely used theory for calculating zeta potential from experimental data is that developed by Marian Smoluchowski in